Fluorescence-based Total Protein Determination using the Compound CBQCA
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چکیده
Total protein content is a measurement common to many applications in basic science and clinical research. Several different fluorescent techniques are available that eliminate many of the problems associated with the traditional colorimetric absorbance-based methods to measure total protein content. Here we describe the use of 3-(4-carboxybenzoyl)quinoline-2carboxaldehyde (CBQCA) to quantitate total protein using the BioTek FL600 fluorescence microplate reader.
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